684 Diff Expressed (2 fold, 0.05) no evalue limit = 10 (this includes no hit

454 up in Barn.
215 up in Mashpee

5 only in Barn
10 only in Mash
Clam_RNAseq_GOslim_mod_1.xlsx

* DEG list
SuppDEG.txt


Clam_RNAseq_GOslim_mod.xlsxis trimmed for evalues 


DAVID analysis (prep)

highest evalue retained .. 0.009 

.01

Left with 140 sequences 

(132 unique SP IDs)






-- side note on de novo transcriptome- 6997 annotated but need to only select low evalues for DAVID.


2746 have evalue max of 9.E-03

487 duplicate SP IDs, 2260 unique SP ID remain.

-----

as for Diff Exp Genes..
See above

data in this fill  V
HardClam DAVID prep.xls



DAVID

notes
these IDs are UniPROT Accession


Gene list
conv_0EE639CBAE631322075013682.txt













g2g_0EE639CBAE631322075241979.txt








chart_0EE639CBAE631322075351830.txt



LIST OF SPIDs that make up enrich..
60 - seems low.

SPIDwithinENRICH.txt

Need to join with DiffExpressed genes to Identify those Enriched Biological Process





chart_0EE639CBAE631322075449997.txt




chart_0EE639CBAE631322075581541.txt




chart_0EE639CBAE631322075635588.txt

-






----

JUST stress response genes. .....
HardClamStressRespone.xlsx

SuppImmune.tab


Comparison based on other Sequencing efforts.... SRA only..l