684 Diff Expressed (2 fold, 0.05) no evalue limit = 10 (this includes no hit
454 up in Barn.
215 up in Mashpee
5 only in Barn
10 only in Mash
* DEG list
DAVID analysis (prep)
highest evalue retained .. 0.009
.01
Left with 140 sequences
(132 unique SP IDs)
-- side note on de novo transcriptome- 6997 annotated but need to only select low evalues for DAVID.
2746 have evalue max of 9.E-03
487 duplicate SP IDs, 2260 unique SP ID remain.
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as for Diff Exp Genes..
See above
data in this fill V
DAVID
notes
these IDs are UniPROT Accession
Gene list
LIST OF SPIDs that make up enrich..
60 - seems low.
Need to join with DiffExpressed genes to Identify those Enriched Biological Process
-
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JUST stress response genes. .....
Comparison based on other Sequencing efforts.... SRA only..l